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Download PDF by Karl Maramorosch: Advances in cell culture. Volume 5

By Karl Maramorosch

ISBN-10: 0120079054

ISBN-13: 9780120079056

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D. (1980). Arthritis Rheum. 23, 1010-1020. Palmoski, M. , and Brandt, K. D. (1983a). Arthritis Rheum. 26, 5 2 8 - 5 3 1 . Palmoski, M. , and Brandt, K. D. (1983b). Arthritis Rheum. 26, 771-774. , Lipman, J. , and Sokoloff, L. (1982a). Arthritis Rheum. 2 5 , 1 2 1 7 1227. , Lipman, J. , McDevitt, C. , and Sokoloff, L. (1982b). Arthritis Rheum. 25, 1228-1238. Reynolds, J. , and Cartwright, E. (1977). Lancet 2, 3 3 3 335. , and Herbage, M. (1985). In "Biology of Invertebrate and Lower Vertebrate Collagens" (A.

Metachromatic clones appeared earlier in HAM F12 (10-14 days) than in DMEM. In the latter, staining was more frequent and more intense. Although the addition of serum permitted a maximal growth rate, cell attachment to culture surfaces, and protection against trypsin activity, the complex composition and variability among the different batches led to the establishment of synthetic serum-free media (SFM) for physiological and pharmacological studies. Moreover, synthetic serum-free media seem to avoid the rapid loss of collagen phenotype expression in culture.

1985). In regard to collagen, Daniel et al. (1984) observed that bovine articular chondrocytes in high-density culture treated with ascorbate secreted an increasing proportion of collagen types I and III into the medium. The ascorbate-treated cells did not incorporate type I collagen into the cell-associated matrix, but continued to accumulate type II collagen in this compartment. Several studies have suggested that vitamin D 3 is involved in cartilage metabolism. , 1980). Harmand et al. (1984) examined the effects of l,25-(OH)2D3 and 24,25-(OH)2D3 on a multilayer culture system for rabbit and human chondrocytes.

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Advances in cell culture. Volume 5 by Karl Maramorosch


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